Illumina sequencing is involved in processing millions of fragments in parallel. Sequencing may be utilized to determine the order of nucleotides in small targeted genomic regions or entire genomes. Select the best tools for your lab.Find popular product groupings for your workflowHighly tunable data output to run virtually any genome, sequencing method, and scale of projectAnalyze the entire genome, focus on regions of interest with whole-exome and targeted sequencing, or study DNA-protein interactions.Deep sequencing enables researchers to assess HIV Type 1 coreceptor usage.Three approaches to help researchers with sequencingA rapid, single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard (non-degraded) RNA.Fast, high-quality, sample-to-data services such as RNA and whole-genome sequencingStudy gene expression changes in cancer, RNA biomarkers of drug response, and moreLibrary preparation options are available for a broad range of sequencing methods, including whole-genome sequencing, whole-exome and targeted sequencing, RNA sequencing, methylation sequencing, and more. The forward strand is washed away, and the process of sequence by synthesis repeats for the reverse strand.Once the DNA strand has been read, the strand that was just added is washed away. For Research Use Only. Nucleotides are distinguished by either one of two colors (red or green), no color ("black") or combining both colors (appearing orange as a mixture between red and green).A polymerase sequences the complementary strand on top of the arched strand. Next-generation sequencing generates masses of DNA sequencing data, and is both less expensive and less time-consuming than traditional Sanger sequencing. The MiniSeq, Illumina's latest benchtop sequencer, enables more cost‐efficient DNA sequencing relative to larger Illumina sequencing platforms (e.g., MiSeq). Deep sequencing refers to sequencing a genomic region multiple times, sometimes hundreds or even thousands of times. High‐throughput sequencing of the 16S rRNA gene on the Illumina platform is commonly used to assess microbial diversity in environmental samples. : Assign map positions and stack breed information for subsequent resequencing to discover single nucleotide polymorphisms (SNPs) and other genetic variations. Then, the index 1 primer attaches, polymerizes the index 1 sequence, and is washed away. These solutions accommodate a variety of throughput needs, from manual protocols to fully automated workstations.Both genome-wide analysis and targeted approaches can provide insight into methylation patterns at a single nucleotide level.Take advantage of a broad range of techniques, from targeted RNA to single-cell and whole-transcriptome sequencing.The user-friendly "Recommended Links" feature allows you to easily find content and products relevant to your specific field of interest. Not for use in diagnostic procedures (except as specifically noted). It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. These resources cover key topics in NGS and are designed to help you plan your first experiment. Illumina dye sequencing is a technique used to determine the series of base pairs in DNA, also known as DNA sequencing.The reversible terminated chemistry concept was invented by Bruno Canard and Simon Sarfati at the Pasteur Institute in Paris. This next-generation sequencing (NGS) approach allows researchers to detect rare clonal types, cells, or microbes comprising as little as 1% of the original sample.
Learn how it compares to conventional methods, and find out how Illumina technology works.Whole-genome shotgun sequencing and transcriptomics provide data to refine drug discovery and developmentExpanded portfolio enables comprehensive genomic profiling from blood and tissueA culture-free method for studying complex microbiomes or environmentsConsidering bringing next-generation sequencing to your lab, but unsure where to start?